Flowjo departmental license12/30/2023 In addition, the biocontainment setup allows sorting and analysis of infectious samples (risk groups 1 and 2) and live human samples. Each sorter unit is equipped with 5 lasers (355 nm, 405nm, 488nm, 561nm and 642nm) and can sort or analyze cells labeled with up to 12 fluorescent antibodies or probes. ICyt-Sony Cell Sorter System : This flow cytometer system contains two separate sorter units housed in a BSL-2 level biosafety cabinet. A site license for FlowJo has been purchased and individual licenses are available to labs for a yearly fee.Ĭurrent instrumentation and equipment include the following systems and analyzers. The workstations in the lab have either Mac-based CellQuest analytical software or PC-based Summit software as well as FlowJo software. ![]() ![]() The core also makes available several workstations and software options for users to analyze their own data at no additional charge. Data can be reanalyzed by staff at the request of the investigator. Standardized data analysis is available to investigators for each set of samples, depending upon the application. In addition, FCIM also offers macrophage/DC generation, T cell expansion and other cellular product and immune assay design services. Immune monitoring services include: peripheral blood cell isolation, plasma/serum isolation, cell banking, cytokine/growth factor analysis (ELISA, Elispot, Luminex) and leukocyte functional assays. Training on analytical cytometers is available to investigators and their staff so that individual laboratories can analyze their own samples at reduced cost.įlow cytometric services include: 1) multiparameter cell phenotyping up to 23 colors on cell analyzers and 12 colors on cell sorters 2) analysis of DNA content and cell cycle 3) analysis of cellular apoptosis and necrosis 4) quantification of cell cytotoxicity 5) intracellular cytokine and protein analysis 6) measurement of cell activation and signal transduction parameters 7) high speed cell sorting of live human and animal cells under BSL-2 biocontainment conditions and 8) single cell sorting into microtiter plates for cloning or single cell sequencing with next generation sequencing (NGS) technologies on campus. Cell sorting and analysis is also available for live human cells and infectious (BSL-2 level) samples. FCIM has been in continuous operation since 1983 and provides a variety of flow cytometric approaches for multiparameter fluorescent analysis and for the sorting of eukaryotic and prokaryotic cell types. More information about the use and function of FlowJo is available at its website.The Flow Cytometry and Immune Monitoring Core (FCIM) provides comprehensive, state-of-the-art analytical flow cytometry cell analysis and high-speed cell sorting services to support both human and animal studies. The development of FlowJo’s graphical user interface made practical the analysis of the extremely voluminous cytometric data.įlowJo became a commercial product in 1996. As the utility and power of cytometers quickly evolved, thereĪrose a need for a more powerful and user-friendly analysis and display program. At that time, the authors' work at Stanford University’s Information Technology departmentīrought them into contact with the Herzenberg Laboratory where modernįlow cytometry originated. Reports when the experiment involves many samples, parameters and/or operations.įlowJo provides tools for the creation of: ![]() Gate to a sample, copy it to a group, and that gate will be automatically placed on all samples in the group.įlowJo's ability to automate repetitive operationsįacilitates the production of statistics tables and graphical Operation on every sample belonging to that group. When one performs an operation on a group, FlowJo performs the same Such as the panel of antibodies with which they are stained, tissue type, or Within a workspace, samples can be grouped or sorted by various attributes Viewing an entire experiment in a Workspace enables you to organize and manage complex cytometry experiments while making detailed graphical reports. In FlowJo samples are organized in a "Workspace" window, which presents a hierarchical view of all the samples and their analyses (gates and statistics). ![]() Hence FlowJo will import and analyze cytometry data regardless of which FACS ( Fluorescence Activated Cell Sorting) machine is Files produced by modern flow cytometers are written in a standard format called. Risk calculators and risk factors for FlowJoįlowJo is the name of a piece of software for analyzing flow cytometry data. US National Guidelines Clearinghouse on FlowJo Articles on FlowJo in N Eng J Med, Lancet, BMJ
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